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STEMCELL Technologies Inc lymphopreptm density gradient medium
Lymphopreptm Density Gradient Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lymphopreptm+density+gradient+medium/lymphopreptm+density+gradient+medium/pm40441082-47-23-27
Average 90 stars, based on 1 article reviews
lymphopreptm density gradient medium - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Saline:

Article Title: Distinct Luteinization Profiles of Cultured Human Granulosa Cells from Small Antral and Preovulatory Follicles.
Article Snippet: For treatment: 100 nM Testosterone (Sigma, T1500-5 g) and/or 25 ng/ mL FSH (Rekovelle, Ferring), or Androstenedione (Merck, Cat: PHL-89562) alone was added to the culture media. .. For washing of the cells, phosphate buffered saline (PBS) (GibcoTM, Life Tech., 1x) and HEPES buffer solution (Gibco, Cat: 15630049) was used. ow nloaded from https://academ ic.oup.com /jcem /advance-article/doi/10.1210/clinem /dgaf218/8106703 by U niversidade Federal da Paraiba (U FPB) user on 22 M ay 2025 LymphoprepTM density gradient medium (StemCell Technologies, Cat: #07851) or Sigma Percoll gradient (Cat: #P4937). .. For RNA isolation; Trizol reagents (Ambion Life Technologies, 15596026), 1-bromo-3-chloropropane (Sigma, B9673-200ML), Ethanol 99%, DEPC H2O and RNeasy minikit 250 (Qiagen, 74106).

Article Title: "Mannose glycans as key players in trained immunity: A novel anti-tumoral catalyst".
Article Snippet: Cell wall glycans isolated from microorganisms are long known to provoke strong immune responses piloted by innate immune cell populations, including monocytes, in the context of Trained Immunity (TI).. However, the contribution of yeast-derived mannan in the reprogramming of monocytes remains ill-defined.. Here, we demonstrated that TI is often accompanied by an altered gene expression profile of selected glycan-binding proteins expressed by monocytes, including DC-SIGN and Dectin-2.

Sterility:

Article Title: Potentiating Antibody-Dependent Cellular Cytotoxicity in Triple-Negative Breast Cancer via the Humanized Anti-CD147 Antibody.
Article Snippet: .. Briefly, 20 mL of heparinized blood was diluted 1:1 with sterile PBS before being overlaid onto LymphoprepTM density gradient medium (STEMCELL Technologies, Col, Vancouver, BC, Germany) in a sterile 50 mL conical tube. ..

Isolation:

Article Title: Mechanisms of cardiomyopathy in duchenne muscular dystrophy: A study using pluripotent stem cells derived from patients with severe and mild motor impairment.
Article Snippet: .. Within 2 hours of collection, peripheral blood mononuclear cells (PBMCs) were isolated using 15 mL SepMateTM tubes (Stemcell Technologies, Cat#15415) and LymphoprepTM density gradient medium (Stemcell Technologies, Cat07851). ..

Article Title: The Elevation and Impact of Peripheral Bile Acids in Chronic Lymphocytic Leukemia
Article Snippet: T-cells were purified from healthy donor peripheral blood lymphocytes (PBLs; obtained from UNMC Elutriation Core) using the EasySepTM Human T-cell Isolation Kit (StemCell Technologies; Vancouver, BC, Canada). .. Peripheral blood mononuclear cells (PBMCs) were isolated from CLL patient blood using LymphoprepTM density gradient medium (StemCell Technologies) according to manufacturer protocols. ..

Article Title: Characterizing biomarkers of ageing in Singaporeans: the ABIOS observational study protocol
Article Snippet: .. PBMCs are isolated from whole blood immediately after blood collection using the LymphoprepTM density gradient medium in SepMateTM PBMC isolation tubes (STEMCELL Technologies Inc., Canada) and cryopreserved in liquid nitrogen until future DNA methylation profiling and immunophenotypic analysis (Supplementary Material, Table ). ..

Article Title: Generation of integration-free induced pluripotent stem cell (iPSC) line MURAi002-A from hemoglobin E/β-thalassemia disease patient harboring β E /β 0 (CD41/42, -CTTT) compound heterozygous mutation.
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from the peripheral blood of a donor with hemoglobin E/β-thalassemia disease by density gradient centrifugation using LymphoprepTM Density Gradient Medium (STEMCELL Technologies). .. Hematopoietic stem/progenitor cells (HSPCs) were isolated from PBMCs by magnetic cell separation (MACS) using CD34 Microbead kit (Miltenyi Biotec) and expanded in StemSpanTM SFEM II medium (Thermo Fisher Scientific) supplemented with 50 ng/mL SCF, 50 ng/mL Flt3-L, 50 ng/mL TPO, 10 ng/mL IL-3, and 10 ng/mL IL-6 (PeproTech) for 3 days.

Purification:

Article Title: Vitamin D 3 -VDR and vitamin A-RAR affect IL-13 and IFNγ secretion from human CD4 + T cells directly and indirectly via competition for their shared co-receptor RXR.
Article Snippet: Funding information LEO Fondet; Danmarks Frie Forskningsfond Abstract The effects of vitamin D and vitamin A in immune cells are mediated through the vitamin D receptor (VDR) and retinoic acid receptor (RAR), respectively.. These receptors share the retinoid X receptor (RXR) cofactor for transcriptional regulation.. We investigated the effects of active vitamin D3 (1,25(OH)2D3) and 9cis retinoic acid (9cRA) on T helper (TH)1 and TH2 cytokines and transcription factors in primary human bloodderived CD4+ T cells.

Gradient Centrifugation:

Article Title: Vitamin D 3 -VDR and vitamin A-RAR affect IL-13 and IFNγ secretion from human CD4 + T cells directly and indirectly via competition for their shared co-receptor RXR.
Article Snippet: Funding information LEO Fondet; Danmarks Frie Forskningsfond Abstract The effects of vitamin D and vitamin A in immune cells are mediated through the vitamin D receptor (VDR) and retinoic acid receptor (RAR), respectively.. These receptors share the retinoid X receptor (RXR) cofactor for transcriptional regulation.. We investigated the effects of active vitamin D3 (1,25(OH)2D3) and 9cis retinoic acid (9cRA) on T helper (TH)1 and TH2 cytokines and transcription factors in primary human bloodderived CD4+ T cells.

Article Title: Generation of integration-free induced pluripotent stem cell (iPSC) line MURAi002-A from hemoglobin E/β-thalassemia disease patient harboring β E /β 0 (CD41/42, -CTTT) compound heterozygous mutation.
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from the peripheral blood of a donor with hemoglobin E/β-thalassemia disease by density gradient centrifugation using LymphoprepTM Density Gradient Medium (STEMCELL Technologies). .. Hematopoietic stem/progenitor cells (HSPCs) were isolated from PBMCs by magnetic cell separation (MACS) using CD34 Microbead kit (Miltenyi Biotec) and expanded in StemSpanTM SFEM II medium (Thermo Fisher Scientific) supplemented with 50 ng/mL SCF, 50 ng/mL Flt3-L, 50 ng/mL TPO, 10 ng/mL IL-3, and 10 ng/mL IL-6 (PeproTech) for 3 days.

DNA Methylation Assay:

Article Title: Characterizing biomarkers of ageing in Singaporeans: the ABIOS observational study protocol
Article Snippet: .. PBMCs are isolated from whole blood immediately after blood collection using the LymphoprepTM density gradient medium in SepMateTM PBMC isolation tubes (STEMCELL Technologies Inc., Canada) and cryopreserved in liquid nitrogen until future DNA methylation profiling and immunophenotypic analysis (Supplementary Material, Table ). ..



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